Background & Aim: Among the biological control agents used in recent years for reducing fungal human diseases, non pathogenic bacteria such as B.subtilis have received major consideration because of their unique ability to produce a wide variety of fungal inhibitory compounds. The purpose of this study was to evaluate the antifungal activity of B.subtilis culture filtration against pathogenic fungi.

Material & Methods: Fifty soil samples were collected from so different part of Tehran and cultured on glucose yeast extract agar for isolate of B.subtilis. All isolated B.subtilis were screened for their ability to inhibit all fungi growth using the dual culture on medium GYA on base visual agar plate assay, then  of B.subtilis culture filtration showed most remarkable antagonistic activites against fungi was prepared serial dilution and antagonistic activity amount was evaluated. The results were analyzed using the SPSS software program.

Results: Totally 38B.subtiliswere obtained from soil samples that showed antagonistic activites against fungi. Bacteria cultured filtrated was able to inhibition more than 50% related to the evaluated fungi. This inhibition amount reported related to the current fungi in comparision to control group using the SPSS software, statistically, less than 0/05 in significant level.

The results showed that there was statistically significant difference between the inhibition amount related to the current fungi compared to the control group (p<0.05)

Conclusions: The results show that the B.subtilis with produce antifungal metabolites can be an appropriate tool for biological control fungi in nature.